| Catalog No. | HB116116 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3 |
| References | 1. Sala G, et al. An ErbB-3 antibody, MP-RM-1, inhibits tumor growth by blocking ligand-dependent and independent activation of ErbB-3/Akt signaling. Oncogene. 2012;31(10):1275-1286. [HB116116] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | MP-RM-1 (0.01-10 ug/mL; 15-120 min) inhibits NRG-1β-dependent phosphorylation of ErbB-3 and Akt in a dose- and time-dependent manner in breast cancer cells MDA-MB-435 and melanoma cells IR-8 [1]. MP-RM-1 (10 ug/mL) significantly inhibits the colony-forming ability of IR-8 melanoma cells [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of ErbB-3 melanoma xenografts in nude mice, and reduces ErbB-3 expression and Akt phosphorylation levels in tumor tissues [1]. MP-RM-1 (20 mg/kg; i.p.; twice weekly) significantly inhibits the growth of DU-145 prostate cancer xenografts in nude mice [1]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P21860 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | MP-RM-1, MPRM1, MPRM1 |
| Clone ID | MP-RM-1 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human ErbB3/Her3 Reference Antibody (MP-RM-1, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human ERBB3/HER3 (MP-RM-1). |
 Flow CytoMetry |
Flow-cytometry using anti-human ERBB3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ERBB3 monoclonal antibody (Catalog: HB116116) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Bioactivity |
Detects recombinant human ERBB3/Her3 (Cat No.: HB116011) in indirect ELISA. |