| Catalog No. | HB997036 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | IL-10, IL10, Cytokine synthesis inhibitory factor, CSIF, Interleukin-10 |
| References | 1. Phan U, et al. Development of the anti-IL-10 mAb MK-1966 in combination with in situ vaccination of a TLR9 agonist SD-101 for cancer immunotherapy[J]. European Journal of Cancer, 2016, 69: S91-S92. [HB997036] 2. Chen J, et al. CD146+ CAFs mediate immunosuppression in gastric cancer via COL4A1: potential therapeutic targets. J Immunother Cancer. 2025;13(12):e013577. Published 2025 Dec 21. [HB997036] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | MK-1966 strongly induces IFNγ production in human PBMCs when combined with the TLR9 agonist SD-101 [1]. MK-1966 (10 ug/mL; 24 h) restores CD8 + T-cell proliferation and functional cytokine production that is suppressed by COL4A1-induced M2-like macrophages [2]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P22301 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | MK-1966, MK1966, MK1966 |
| Clone ID | MK-1966 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human IL-10 Reference Antibody (MK-1966, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human IL10 (MK-1966) |
 Flow cytometry |
Flow-cytometry using anti-human IL10 antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL10 monoclonal antibody (Catalog HB997036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |