| Catalog No. | HV556026 |
| Brand | abinScience |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | IL-1R3, C3orf13, Interleukin-1 receptor 3, Interleukin-1 receptor accessory protein, IL1RAP, IL-1R-3, IL-1 receptor accessory protein, IL1R3, IL-1RAcP |
| References | 1. Grönberg C, et al. Combined inhibition of IL-1, IL-33 and IL-36 signalling by targeting IL1RAP ameliorates skin and lung fibrosis in preclinical models of systemic sclerosis. Ann Rheum Dis. 2024;83(9):1156-1168. Published 2024 Aug 27. [HV556026] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | CAN10 effectively impairs the proinflammatory and profibrotic effects of IL1RAP-related cytokines (IL-1, IL-33, and IL-36) in human umbilical cord endothelial cells and human dermal fibroblasts, which are critical to systemic sclerosis pathophysiology [1]. CAN10 (mCAN10, mouse surrogate of CAN10) (10-20 mg/kg; i.p.; twice weekly; 7 weeks) reduces cutaneous and pulmonary fibrosis in a murine cGvHD model of systemic sclerosis, with significant improvements in dermal thickness, hydroxyproline content, myofibroblast counts, and lung Ashcroft scores [1]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q9NPH3 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | CAN10 |
| Clone ID | CAN10 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human IL-1 RAcP/IL-1 R3 Reference Antibody (CAN10, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-Human IL1RAP Reference Antibody (CAN10, RUO) |
 Bioactivity |
Detects Recombinant Human IL1RAP Protein, N-His (Catalog No: HV556011) in indirect ELISAs. |
 Western blot |
Western blot analysis was performed using anti-IL1RAP monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human IL1RAP (Catalog No: HV556011) Lane 2: negative control Lane 3: negative control |