Anti-Human IL-1 beta/IL-1F2 Reference Antibody (SK48-E26, RUO)

Catalog No.	HF943086
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta
References	1. Peter Ronald Young, et al. RECOMBINANT AND HUMANIZED IL-1β ANTIBODIES FOR TREATMENT OF IL-1 MEDIATED INFLAMMATORY DISORDERS IN MAN. WO1995001997A1. 1994. [HF943086] 2. Lacy SE, et al. Generation and characterization of ABT-981, a dual variable domain immunoglobulin (DVD-Ig(TM)) molecule that specifically and potently neutralizes both IL-1α and IL-1β. MAbs. 2015;7(3):605-19. [HF943086]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	SK48-E26 effectively captures human recombinant IL-1β in an ELISA format, enabling detection of hrIL-1β at concentrations as low as 0.1 to 0.2 ng/mL with a linear response to IL-1β concentration [1]. SK48-E26 specifically neutralizes human recombinant IL-1β activity in an EL-4 cell-based bioassay, binds a conformational epitope on hrIL-1β spanning amino acids 95-101, and has an affinity for IL-1β of 0.5 to 3 nM [1]. Mutations in the heavy chain CDR3 region of SK48-E26 Fab fragments increase affinity for human recombinant IL-1β, with the V120T/R121K mutant showing a 9.79-fold improvement over the wild-type SK48-E26 Fab [1]. SK48-E26 (10-200 nM) binds to human IL-1β with an equilibrium dissociation constant of 242 pM [2]. SK48-E26 (1×10 -15 -1×10 -7 M; 1 h pre-incubation with IL-1β, 16-20 h incubation with cells) neutralizes human IL-1β with an IC 50 of 400 pM and cynomolgus IL-1β with an IC 50 of 185 pM in MRC-5 cells [2].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P01584
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	SK48-E26, SK48E26, SK48E26
Clone ID	SK48-E26
Note	For research use only. Not suitable for clinical or therapeutic use.