Anti-Human IL-2 Reference Antibody (MT204, RUO)

Catalog No.	HX011046
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
References	1. Plater-Zyberk C, et al. The interleukin-2 antagonizing antibody MT204 delays allogeneic skin graft rejection in non-human primates and is well tolerated. Transpl Immunol. 2011 Sep; 25(2-3):133-40. [HX011046] 2. Volkland J, et al. A humanized monoclonal antibody against interleukin-2 that can inactivate the cytokine/receptor complex. Mol Immunol. 2007 Mar; 44(7):1743-53. [HX011046]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	MT204 (1-100 ug/mL, 4 days) dose-dependently inhibits proliferation and IFN-γ secretion in rhesus PBMCs stimulated by the mitogen concavalin A [1]. MT204 can block CD25-bound IL-2 on high-affinity IL-2 receptors with a k d of 0.58 nM [1] [2]. MT204 (5 h) dose dependently reduces the IL-2 inducible cell surface expression of the alpha subunit of IL-4 receptor (CD124) in CTLL-2 cells [2]. MT204 (5 days) inhibits IL-2-induced proliferation of NKL cells and T cells with IC 50 s of approximately 1.3 nM and 0.21 nM, respectively [2]. MT204 (15 min) potently antagonizes downstream signaling of IL-2 at sub-nanomolar concentrations, significantly reducing cellular pY-STAT3 levels stimulated by IL-2 in Primary human lymphocytes [2]. MT204 significantly inhibits IL-2 induced proliferation independently of CD25 expression levels in NKL cells, T cells and all NK cells with IC 50 s of 0.35-0.36 nM, 0.33 nM, and 0.1-0.13 nM, respectively [2]. Parmacokinetics Species Dose Route Note Plasma Concentration T 1/2 Rhesus monkey 2, 10, 50 or repeat 50 mg/kg i.v. 1 h infusion 1 mg/mL 326.4 h MT204 (50 mg/kg, i.v., day 0, day 5 and day 12 at a speed of 1.5 mL/kg/h over 1 h) significant delays in rejection of allogeneic skin graft in allogeneic skin graft model of rhesus monkey [1].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P60568
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	MT204
Clone ID	MT204
Note	For research use only. Not suitable for clinical or therapeutic use.