| Catalog No. | HY112036 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | INSR, IR, CD220, Insulin receptor |
| References | 1. Cieniewicz AM, et al. Novel Monoclonal Antibody Is an Allosteric Insulin Receptor Antagonist That Induces Insulin Resistance. Diabetes. 2017 Jan;66(1):206-217. [HY112036] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P06213 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | IRAB-B, IRABB, IRABB |
| Clone ID | IRAB-B |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human Insulin R/CD220 Reference Antibody (IRAB-B, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD220/INSR (IRAB-B) |
 Flow cytometry |
Flow-cytometry using anti-human CD220 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD220 monoclonal antibody (Catalog HY112036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |