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Anti-Human OSM/Oncostatin-M Reference Antibody (GSK315234, RUO)
Overview
Catalog No. HB745026
Brand abinScience
Host species Human
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target Oncostatin-M, OSM
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P13725
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameGSK315234
Clone IDGSK315234
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Western blot
Western blot analysis was performed using anti-OSM monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OSM (Catalog No: HB745011)
Lane 2: negative control

Bioactivity
Detects recombinant human OSM/Oncostatin-M (Catalog No: HB745011) in indirect ELISA

Flow cytometry
Flow-cytometry using anti-human OSM antibody. KG-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human OSM monoclonal antibody (Catalog HB745026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.