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Anti-Human P-Cadherin Reference Antibody (FF-21101, RUO)
Overview
Catalog No. HB245026
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target Placental cadherin, CDH3, Cadherin-3, P-cadherin, CDHP
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P22223
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameFF-21101(90Y), FF21101(90Y), FF21101(90Y)
Clone IDFF-21101
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Bioactivity
SEC-HPLC detection for Research Grade Anti-Human CDH3/P-cadherin (FF-21101).

SDS-PAGE
SDS-PAGE for Research Grade Anti-Human CDH3/P-cadherin (FF-21101).

Flow CytoMetry
Flow-cytometry using APC anti-human CDH3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CDH3 monoclonal antibody (Catalog: HB245026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human CDH3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CDH3 monoclonal antibody (Catalog: HB245026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using anti-human CDH3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CDH3 monoclonal antibody (Catalog: HB245026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.