Anti-Human Transferrin R/TfR Reference Antibody (JST-TFR09, RUO)

Catalog No.

HY036096

Brand

abinScience

Tested applications

FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Isotype

IgG1

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Target

Transferrin receptor protein 1, TfR1, T9, Trfr, p90, CD71, TFRC, TfR, sTfR, TR

References

1. Fauzi YR, et al. Anti-transferrin receptor antibody (JST-TFR09/PPMX-T003) induces ferroptosis in adult T-cell leukemia/lymphoma (ATLL) cells. Biochem Biophys Res Commun. 2025;756:151564. [HY036096]
2. Daniels-Wells TR, et al. Efficacy of Antibodies Targeting TfR1 in Xenograft Mouse Models of AIDS-Related Non-Hodgkin Lymphoma. Cancers (Basel). 2023;15(6):1816. Published 2023 Mar 17. [HY036096]

Purification

Protein A/G purified from cell culture supernatant.

Biological activity

JST-TfR09 (10 ng/well-200 ng; 48 h) reduces viability of MT2, Su9T1, and KK1, and this cell death is inhibited by ferrostatin-1 [1]. JST-TfR09 (100 ng/mL; 48 h) downregulates TFR1, FTH1, and FTL, upregulates IRP2 and NCOA4, and induces lysosome-dependent ferritin degradation and autophagy in ED, KK1, and MT2 ATLL cell lines [1]. JST-TfR09 (100 ng/mL; 48 h) increases intracellular ferrous iron, ROS, and lipid peroxidation (MDA) levels, key hallmarks of ferroptosis, in Su9T1, MT2, ST1, SO4, KK1, and ED ATLL cell lines and MOLT4, JURKAT T-ALL cell lines [1]. JST-TfR09 induces in vitro toxicity to human erythroblasts differentiated from CD34 hematopoietic stem cells at concentrations greater than 156 ng/mL [2]. . Increased cell viability to almost 80% in MT2, Su9T1, and KK1 ATLL cell lines when co-treated with ferrostatin-1. Western Blot Analysis [1] Cell Line: ATLL cell lines (ED, KK1, MT2) Concentration: 100 ng/mL; 25 uM Aloxistatin () + 50 uM Pepstatin A () (pretreatment); 10 uM MG132 () (pretreatment) Incubation Time: 48 h (JST-TfR09); 24 h (pretreatment prior to JST-TfR09) Result: Decreased TFR1, FTH1, and FTL protein levels in ED and KK1 cells. Increased IRP2 and NCOA4 protein levels in ED and KK1 cells. Prevented JST-TfR09-induced ferritin degradation in cells pretreated with lysosomal inhibitors, while proteasomal inhibitor pretreatment did not. Increased LC3-II expression relative to LC3-I in cells, indicating autophagy activation, which was blocked by lysosomal inhibitor co-treatment.

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

P02786

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

Alternative name

JST-TFR09, JSTTFR09, JSTTFR09

Clone ID

JST-TFR09

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only. Not suitable for clinical or therapeutic use.

RRID

Anti-Human Transferrin R/TfR Reference Antibody (JST-TFR09, RUO) (abinScience Cat# HY036096, RRID:AB_3751763)

Isotype Control

Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)

SDS-PAGE	SDS-PAGE for Research Grade Anti-Human CD71/TFRC (JST-TFR09).
Flow CytoMetry	Flow-cytometry using APC anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.