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Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Overview
Catalog No. HY057043
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype VHH-His
Applications ELISA, FCM
Target T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Purification Purified by Nickel column.
Endotoxin level Please contact with the lab for this information.
Accession P07766
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA2242
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)

Bioactivity
Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.

Bioactivity
Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.

Flow cytometry
Flow cytometry analysis was performed using anti-human CD3 monoclonal antibody. Jurkat cells were incubated with an irrelevant antibody (blue histogram) or FITC-conjugated anti-human CD3 monoclonal antibody (Catalog # HY057043, green histogram) at a concentration of 5 µg/mL for 30 min at room temperature. After washing, cells were directly analyzed on a NovoCyte Flow Cytometer.