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Mouse Anti-DNA Recombinant AutoRecombinant Antibody (PME77)
Overview
Catalog No. YP373073
Brand abinScience
Species reactivity Mouse
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG2a, lambda
Applications ELISA, WB, IF
Target dsDNA
Purification Protein A/G purified from cell culture supernatant.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Clone IDPME77
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Note For research use only.
RRID Mouse Anti-DNA Recombinant Autoantibody (PME77) (abinScience Cat# YP373073, RRID:AB_3729874)
Images


SDS-PAGE
SDS-PAGE for Mouse Anti-DNA Recombinant Autoantibody (PME77)