Anti-Human PD-L1 & TGF-beta RII Reference Antibody (Retlirafusp Alfa, RUO)

Catalog No.

HV974406

Brand

abinScience

Host species

Human

Tested applications

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG4-kappa

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Target

B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274, TGFR-2, TbetaR-II, TGF-beta type II receptor, TGFBR2, TGF-beta receptor type-2, TGF-beta receptor type II, Transforming growth factor-beta receptor type II

References

1. Bo Cheng, et al. Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer. Cancer Commun (Lond). 2022 Jan;42(1):17-36. [HV974406]

Purification

Protein A/G purified from cell culture supernatant.

Biological activity

SHR-1701 (10 ug/mL) can inhibit TGF-β signaling pathway, activate PI3K/Akt/Erk signaling pathway, rescue lymphocytes and restore the anti-tumor function of peripheral blood CD8 + T cells in patients with lung cancer [1].

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

Q9NZQ7 & P37173

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

SHR-1701

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only. Not suitable for clinical or therapeutic use.

SDS-PAGE	SDS-PAGE for Research Grade Retlirafusp Alfa.
Bioactivity	Detects recombinant PDL1 (Catalog No: HV974011) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-PDL1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human PDL1 (Catalog No: HV974011) Lane 2: Negative control