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Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Overview
Catalog No. HF996136
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P01589
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name2733581-99-0
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Flow CytoMetry		 Flow-cytometry using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD25 monoclonal antibody (Catalog: HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry APC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry PE using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD25 monoclonal antibody (Catalog HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.