Anti-Human BCMA/TNFRSF17 & CD3 epsilon Reference Antibody (Vonsetamig, RUO)

Catalog No.

HX959196

Brand

abinScience

Tested applications

WB, FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Isotype

IgG4-kappa

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Target

Tumor necrosis factor receptor superfamily member 17, TNFRSF17, CD269, B-cell maturation protein, BCMA, BCM, T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

Q02223 & P07766

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

2408319-33-3

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107)

SDS-PAGE	SDS-PAGE for Research Grade Vonsetamig.
Flow cytometry	Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E monoclonal antibody (Catalog HX959196, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using anti-human CD269 antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD269 monoclonal antibody (Catalog HX959196, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity	Detects recombinant CD269 (Catalog No: HX959012) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-CD3E monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD3E (Catalog No: HY057021) Lane 2: Negative control