Anti-sLea/CA19-9 Reference Antibody (MVT-5873, RUO)

Catalog No.	HP481016
Brand	abinScience
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CA19-9, Sialyl Lewis A, sLe A
References	1. Ugorski M, et al. Sialyl Lewis(a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells. Acta Biochim Pol. 2002;49(2):303-311. [HP481016] 2. Takada A, et al. Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium. Cancer Res. 1993 Jan 15;53(2):354-61. [HP481016] 3. Ma Z, et al. Expression of the Carbohydrate Lewis Antigen, Sialyl Lewis A, Sialyl Lewis X, Lewis X, and Lewis Y in the Placental Villi of Patients With Unexplained Miscarriages. Front Immunol. 2021;12:679424. Published 2021 May 31. [HP481016]
Purification	Protein A/G purified from cell culture supernatant.
Biological activity	Sialyl Lewis A mediates adhesion of human colon, pancreas, and gastric cancer cells to E-selectin-expressing endothelial cells, with complete abolition of this adhesion when Sialyl Lewis A expression is suppressed via antisense FT III cDNA transfection [1]. Sialyl Lewis A mediates adhesion of human urinary bladder cancer Hu 1703He cells to E-selectin-expressing CHO cells [1]. Sialyl Lewis A expression is required for the tumorigenicity of human squamous lung cancer cells in athymic nu/nu nude mice [1]. Sialyl Lewis A mediates nearly exclusive ELAM-1-dependent adhesion of Capan2, 2102Ep, QG90, Colo201, WiDr, and SW1116 human epithelial cancer cell lines to rIL1β-activated HUVECs [2]. Sialyl Lewis A is strongly expressed on the surface of Capan2, 2102Ep, QG90, Colo201, WiDr, and SW1116 human epithelial cancer cell lines, but is not detected on any of the 12 tested human leukemia cell lines [2]. Sialyl Lewis A does not contribute to the adhesion of any of the 12 tested human leukemia cell lines to rIL1β-activated HUVECs [2].
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	HuMab-5B1, MVT-5873
Clone ID	MVT-5873
Note	For research use only. Not suitable for clinical or therapeutic use.