| Catalog No. | HC598016 |
| Brand | abinScience |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | CLEC5B, KLRB1, CD161, HNKR-P1a, C-type lectin domain family 5 member B, NKRP1A, Natural killer cell surface protein P1A, Killer cell lectin-like receptor subfamily B member 1, NKR-P1A |
| References | 1. Zhao Y, et al. Structure-Based Discovery of 4-(6-Methoxy-2-methyl-4-(quinolin-4-yl)-9H-pyrimido[4,5-b]indol-7-yl)-3,5-dimethylisoxazole (CD161) as a Potent and Orally Bioavailable BET Bromodomain Inhibitor. J Med Chem. 2017 May 11;60(9):3887-3901. [HC598016] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Biological activity | CD161 has K i s of 8.2 nM and 1.4 nM for BRD4 BD1 and BRD4 BD2, respectively [1]. CD161 (30-3000 nM; 1 hours) is very effective in inducing rapid down-regulation of c-Myc at as early as the 1 h time point and in a dose-dependent manner [1]. Western Blot Analysis [1] Cell Line: MV4;11 leukemia cells. Concentration: 30, 100, 300, 1000, 3000 nM Incubation Time: 1 hours Result: Induced rapid down-regulation of c-Myc at as early as the 1 hours time point and in a dose-dependent manner. CD161 (po; 20, 40 mg/kg/day; 45 days) achieves essentially complete tumor growth inhibition [1]. CD161 (5 mg/kg (iv), 25 mg/kg (po); 0-24 hours) has the t 1/2 of 2.4 hours (iv) and 2.9 hours (po) for rat; the C max of 7333 ng/mL (po) for rat. The t 1/2 of mice is 0.5 hours (iv) and 1.60 hours (po); the C max of mice is 983.1 ng/mL (po) [1]. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q12918 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | 2762201-86-3 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human CD161 Reference Antibody (Rezorstobart, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Rezorstobart |
 Bioactivity |
Detects recombinant human CD161 (Catalog No: HC598021) in indirect ELISA |