Anti-Human 4-1BB/TNFRSF9/CD137 & PD-L1 Bispecific Reference Antibody (Xirestomig, RUO)

Catalog No.

HC336176

Brand

abinScience

Host species

Human

Tested applications

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG1-scFv_L2 dimer

Applications

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

Target

4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9, B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg as determined by LAL method.

Accession

Q07011 & Q9NZQ7

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

Bispecific, 2756879-35-1

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only. Not suitable for clinical or therapeutic use.

SDS-PAGE	SDS-PAGE for Research Grade Xirestomig.
Bioactivity	Detects recombinant CD137 (Catalog No: HC336011) in indirect ELISA.
Bioactivity	Detects recombinant PDL1 (Catalog No: HV974011) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-PDL1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human PDL1 (Catalog No: HV974011)
Immunofluorescence	Immunofluorescence staining of HDLM2 with Human monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.
Immunofluorescence	Immunofluorescence staining of HEK293T cells overexpressing PD-L1 with Human monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.