| Catalog No. | HY196206 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa/G1-lambda |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET, HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P08581 & P00533 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, 2868265-50-1 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Tilatamig, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Tilatamig. |
 Bioactivity |
Detects recombinant human EGFR/ERBB1/HER1 (Catalog No: HF004011) in indirect ELISA |
 Flow cytometry |
Flow-cytometry using anti-human MET antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MET monoclonal antibody (Catalog HY196206, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using anti-human EGFR antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR monoclonal antibody (Catalog HY196206, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |