Anti-Human ErbB2/Her2 & 4-1BB/TNFRSF9/CD137 Bispecific Reference Antibody (Cinrebafusp Alfa, RUO)

Catalog No.

HC336186

Brand

abinScience

Host species

Human

Tested applications

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG

Applications

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

Target

CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg as determined by LAL method.

Accession

P04626 & Q07011

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207)

SDS-PAGE	SDS-PAGE for Research Grade Cinrebafusp alfa.
Bioactivity	Detects recombinant CD340 (Catalog No: HY286011) in indirect ELISA.
Bioactivity	Detects recombinant CD137 (Catalog No: HC336011) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD137 (Catalog No: HC336011) Lane 2: Negative control
Immunofluorescence	Immunofluorescence staining of HDLM2 with Human monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.