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Anti-Human MSLN/Mesothelin Recombinant Antibody (SAA0145)
Overview
Catalog No. HC496107
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, lambda
Applications FCM, IHC
Target CAK1 antigen, MPF, MSLN, Megakaryocyte-potentiating factor, Mesothelin, Mesothelin, cleaved form, Pre-pro-megakaryocyte-potentiating factor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession Q13421
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA0145
Note For research use only.
Images


Flow Cytometry
Flow-cytometry using anti-human MSLN antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MSLN antibody monoclonal antibody (Catalog # HC496107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using anti-human MSLN antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MSLN monoclonal antibody (Catalog: HC496107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.