| Catalog No. | HF004107 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM, WB |
| Target | EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Accession | P00533 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | 11F8 |
| Note | For research use only. |
Anti-Human EGFR Recombinant Antibody (11F8)
Overview
Images
 Flow-cytometry |
Flow-cytometry using anti-human EGFR antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human EGFR antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human EGFR antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # HF004107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |