| Catalog No. | HY378107 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa slience |
| Applications | ELISA, FCM, IHC, WB |
| Target | CD18, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta, ITGB2, Integrin beta-2, MFI7 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Accession | P05107 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | SAA0815 |
| Note | For research use only. |
Anti-Human Integrin beta 2/CD18 Recombinant Antibody (SAA0815)
Overview
Images
 Flow-cytometry |
Flow-cytometry using anti- human CD18 antibody. Human U-937cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti- human CD18 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Bioactivity |
SEC-HPLC detection for Anti-Human CD18/ITGB2 Antibody (ABS0955). |
 Flow CytoMetry |
Flow-cytometry using anti-human CD18 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD18 monoclonal antibody (Catalog: HY378107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |