| Catalog No. | HB011307 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM |
| Target | Sialic acid-binding Ig-like lectin 2, Siglec-2, CD22, T-cell surface antigen Leu-14, BL-CAM, SIGLEC2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22, RFB4, RFB 4 |
| References | 1. Kreitman RJ, et al. Complete regression of human B-cell lymphoma xenografts in mice treated with recombinant anti-CD22 immunotoxin RFB4(dsFv)-PE38 at doses tolerated by cynomolgus monkeys. Int J Cancer. 1999 Mar 31;81(1):148-55. [HB011307] 2. Mansfield E, et al. Recombinant RFB4 immunotoxins exhibit potent cytotoxic activity for CD22-bearing cells and tumors. Blood. 1997;90(5):2020-2026. [HB011307] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P20273 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | RFB-4 |
| Note | For research use only. |
Anti-Human Siglec-2/CD22 Recombinant Antibody (RFB-4)
Overview
Images
 Bioactivity |
Detects CD22 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CD22 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD22 antibody monoclonal antibody (Catalog # HB011307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |