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Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#)
Overview
Catalog No. HB102107
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM, IHC, WB
Target Breast carcinoma-associated antigen DF3, CA 15-3, CD227, Cancer antigen 15-3, Carcinoma-associated mucin, EMA, Episialin, H23AG, KL-6, Krebs von den Lungen-6, MUC-1, MUC1, MUC1-CT, MUC1-NT, MUC1-alpha, MUC1-beta, Mucin-1, Mucin-1 subunit alpha, Mucin-1 subunit beta, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P15941
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID5E5#
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human MUC1-Tn/STn Antibody (5E5#).

SDS-PAGE
SDS-PAGE for Anti-Human MUC1-Tn/STn Antibody (5E5#)

Flow CytoMetry
Flow-cytometry using anti-human CD227 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD227 monoclonal antibody (Catalog: HB102107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.