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Anti-Human CD276/B7-H3 Recombinant Antibody (Mab 8H9)
Overview
Catalog No. HV612207
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM
Target 4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession Q5ZPR3
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDMab 8H9
Note For research use only.
Images


Flow Cytometry
Flow-cytometry using anti-human CD276 antibody.293T cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD276 antibody monoclonal antibody (Catalog # HV612207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human CD276 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD276 antibody monoclonal antibody (Catalog # HV612207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.