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Anti-Human EpCAM/TROP1 Recombinant Antibody (MT201)
Overview
Catalog No. HB104107
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM
Target Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P16422
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDMT201
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human CD326/EPCAM Antibody (MT201)

Flow Cytometry
Flow-cytometry using anti-human CD326 antibody.CD326 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 antibody monoclonal antibody (Catalog # HB104107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human CD326 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 antibody monoclonal antibody (Catalog # HB104107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using anti-human CD326 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD326 monoclonal antibody (Catalog: HB104107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.