| Catalog No. | HF802016 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P01583 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | MABp1, Xilonix, CAS: 1401965-15-8, CA-18C3, CV-18C3, RA-18C3, T2-18C3 |
| Species | Human |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human IL-1 alpha/IL-1F1 Reference Antibody (Bermekimab, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Bermekimab. |
 Bioactivity |
Detects IL1A/IL1F1 in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Bermekimab. |
 Flow cytometry |
Flow-cytometry using PE anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using FITC anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog HF802016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |