Anti-Human IL-1 alpha/IL-1F1 Reference Antibody (Lutikizumab, RUO)

Catalog No.	HF802026
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% as determined by SDS-PAGE.
Isotype	IgG1-kappa (DVD-Ig)
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha, Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P01583 & P01584
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	Bispecific, ABT-981, 1791411-57-8
Species	Human
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	Detects IL1A/IL1F1 in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Lutikizumab.
Flow cytometry	Flow-cytometry using APC anti-human IL1A&IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human IL1A&IL1B monoclonal antibody (Catalog HF802026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using anti-human IL1A&IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A&IL1B monoclonal antibody (Catalog HF802026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.