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Anti-Human CD47 Recombinant Antibody (5F9-G4)
Overview
Catalog No. HC359107
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG4, kappa
Applications FCM
Target Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession Q08722
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID5F9-G4
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human CD47/MER6 Antibody (5F9-G4).

Flow Cytometry
Flow-cytometry using anti-human CD47 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 monoclonal antibody (Catalog HC359107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.