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Anti-Human Transferrin R/TfR Reference Recombinant Antibody (JR-141)
Overview
Catalog No. HY036107
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM
Target CD71, T9, TFRC, TR, TfR, TfR1, Transferrin receptor 1, Transferrin receptor protein 1, Transferrin receptor protein 1, serum form, Trfr, p90, sTfR
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P02786
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDJR-141
Note For research use only.
Images


Flow-cytometry
Flow-cytometry using anti-human CD71 antibody.CD71 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry
Flow-cytometry using anti-human CD71 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Bioactivity
SEC-HPLC detection for Anti-Human CD71/TFRC Antibody (JR-141).

Flow CytoMetry
Flow-cytometry using anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 monoclonal antibody (Catalog: HY036107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.