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Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034)
Overview
Catalog No. HB209307
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM
Target 5'-NT, 5'-deoxynucleotidase, 5'-nucleotidase, CD73, EC:3.1.3.35, EC:3.1.3.5, EC:3.1.3.89, EC:3.1.3.91, EC:3.1.3.99, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, NT5, NT5E, NTE, Thymidylate 5'-phosphatase
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P21589
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA0034
Note For research use only.
Images


Bioactivity
SEC-HPLC detection for Anti-Human CD73/NT5E Antibody (ABS1815).

Flow Cytometry
Flow-cytometry using anti-human CD73 antibody.U87 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD73 antibody monoclonal antibody (Catalog # HB209307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.