| Catalog No. | HS856036 |
| Brand | abinScience |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17A, IL17A, CTLA8, IL-17, Interleukin-17A, IL17F, Interleukin-17F, IL-17F, Cytokine ML-1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q16552 & Q96PD4 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | CDP4940, UCB-4940, UCB4940, 1418205-77-2 |
| Species | Human |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human IL-17A/F Heterodimer Reference Antibody (Bimekizumab, RUO)
Overview
Images
 Bioactivity |
Detects IL17F in indirect ELISAs. |
 SEC-HPLC |
SEC-HPLC detection for Research Grade Bimekizumab. |
 Bioactivity |
Detects Human IL17A in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human IL17A&IL17F antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL17A&IL17F monoclonal antibody (Catalog HS856036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Research Grade Bimekizumab |
 Western blot |
Western blot analysis was performed using anti-IL17A monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL17A (Catalog No: HS856011), 50ng Lane 2: recombinant human IL17A (Catalog No: HS856011), 30ng Lane 3: recombinant human IL17A (Catalog No: HS856011), 20ng Lane 4: recombinant human IL17A (Catalog No: HS856011), 10ng |