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Anti-Human CD8 alpha Recombinant Antibody (OKT8)
Overview
Catalog No. HF815207
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications in vivo CD8+ T cell depletion in humanized mice
Target T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 1 EU/mg as determined by LAL method.
Accession P01732
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDOKT8
Note For research use only
Images


Flow-cytometry
Flow-cytometry using anti-human CD8a antibody.CD8a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD8a antibody monoclonal antibody (Catalog # HF815207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Human CD8a/Lyt2 Antibody (OKT8)