| Catalog No. | MC336207 |
| Brand | abinScience |
| Host species | Rat |
| Tested applications | FCM |
| Species reactivity | Mouse |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | in vivo 4-1BB stimulation, in vitro 4-1BB stimulation |
| Target | 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Accession | P20334 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | 3H3 |
| Note | For research use only. |
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (3H3)
Overview
Images
 Bioactivity |
Detects CD137/4-1BB/TNFRSF9 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-mouse CD137 antibody.CD137/4-1BB/TNFRSF9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD137 antibody monoclonal antibody (Catalog # MC336207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer. |
 Immunofluorescence |
Immunofluorescence staining of HDLM2 with Rat monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane. |