| Catalog No. | HW776023 | ||||
| Brand | abinScience | ||||
| Tested applications | WB, FCM | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Isotype | IgG1, kappa | ||||
| Applications | ELISA, FCM, IP | ||||
| Target | T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2 | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Accession | P42081, K7GMU1 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||
| Clone ID | 3D1 | ||||
| Product Usage Information |
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| Note | For research use only | ||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
Anti-Human B7-2/CD86 Recombinant Antibody (3D1)
Overview
Images
 Western blot |
Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD86/B7-2 (Catalog No: HW776011) Lane 2: negative control |
 Flow cytometry |
Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 monoclonal antibody (Catalog HW776023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer. |