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Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)
Overview
Catalog No. MC336023
Brand abinScience
Host species Mouse
Tested applications WB
Species reactivity Mouse
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications ELISA, WB
Target 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P20334
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA2312
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Note For research use only
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images


Western blot
Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse CD137 (Catalog No: MC336012)
Lane 2: negative control

Immunofluorescence
Immunofluorescence staining of HDLM2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.