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Anti-Human EpCAM/TROP1 Recombinant Antibody (C52)
Overview
Catalog No. HB104307
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications ELISA, FCM, IF, IHC, IP, SPR, WB
Target Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P16422
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDC52
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human EPCAM Antibody (C52)

Bioactivity
Detects recombinant human CD326/EPCAM (Catalog No: HB104011) in indirect ELISA

Flow cytometry
Flow-cytometry using anti-human CD326 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 monoclonal antibody (Catalog HB104307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.