下载
Anti-Mouse PD-L1 Recombinant Antibody (SAA0359)
Overview
Catalog No. MV974207
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Mouse
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype VHH-mFc (IgG2a)
Applications ELISA, FCM
Target B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg as determined by LAL method.
Accession Q9EP73
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA0359
Note For research use only.
Images


Bioactivity
Detects CD274/PD-L1/B7-H1 in indirect ELISAs.

Flow CytoMetry
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: MV974207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody (SAA0359)