| Catalog No. | HF802107 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM |
| Target | IL1F1, Interleukin-1 alpha, IL1A, Hematopoietin-1, IL-1 alpha |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01583 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | SAA0366 |
| Note | For research use only. |
Anti-Human IL-1 alpha/IL-1F1 Recombinant Antibody (SAA0366)
Overview
Images
 Flow-cytometry |
Flow-cytometry using anti-human IL1A antibody.IL1A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human IL1A antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human IL1A antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A antibody monoclonal antibody (Catalog # HF802107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using anti-human IL1A antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1A monoclonal antibody (Catalog HF802107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer. |