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Anti-Human IL-13 Recombinant Antibody (SAA0382)
Overview
Catalog No. HW632107
Brand abinScience
Host species Mouse
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM, WB
Target IL-13, IL13, Interleukin-13, NC30
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession P35225
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA0382
Note For research use only.
Images


Bioactivity
Detects Human IL13 in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human IL13 antibody.IL13 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL13 antibody monoclonal antibody (Catalog # HW632107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-IL13 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL13 (Catalog No: HW632012)
Lane 2: negative control