| Catalog No. | HB996053 | ||||
| Brand | abinScience | ||||
| Host species | Human | ||||
| Tested applications | FCM | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG1, kappa | ||||
| Applications | ELISA, FCM, Functional assay | ||||
| Target | B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19, Differentiation antigen CD19, T-cell surface antigen Leu-12 | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | < 10 EU/mg | ||||
| Accession | P15391 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Clone ID | HB12B | ||||
| Product Usage Information |
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| Note | For research use only | ||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human CD19 Recombinant Antibody (HB12B)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-Human CD19 Antibody (HB12B) |
 Flow cytometry |
Flow-cytometry using anti-human CD19 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD19 monoclonal antibody (Catalog HB996053, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |