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Anti-Human Desmoglein-3 Recombinant Antibody (AK23)
Overview
Catalog No. HW575013
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications IP, IF, ELISA
Target Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6, DSG3, CDHF6
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P32926
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDAK23
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
Note For research use only
Isotype Control Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
Images


SDS-PAGE
SDS-PAGE for Anti-Human DSG3 Antibody (AK23)

Flow cytometry
Flow-cytometry using anti-human Desmoglein 3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Desmoglein 3 monoclonal antibody (Catalog # HW575013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SEC-HPLC
SEC-HPLC detection for Anti-Human DSG3 Antibody (AK23).