下载
Anti-Human TRAIL R2/TNFRSF10B Recombinant Antibody (SAA1413)
Overview
Catalog No. HT348307
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, lambda
Applications FCM
Target CD262, DR5, Death receptor 5, KILLER, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL receptor 2, TRAIL-R2, TRAILR2, TRICK2, Tumor necrosis factor receptor superfamily member 10B, ZTNFR9
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession O14763
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDSAA1413
Note For research use only.
Images


Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.CD262 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.HUVEC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry
Flow-cytometry using anti-human CD262 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD262 antibody monoclonal antibody (Catalog # HT348307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Bioactivity
SEC-HPLC detection for Anti-Human CD262/TNFRSF10B/TRAIL-R2 Antibody (ABS1916).

Flow CytoMetry
Flow-cytometry using anti-human CD262 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD262 monoclonal antibody (Catalog: HT348307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.