| Catalog No. | HY286307 |
| Brand | abinScience |
| Host species | Mouse |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | ELISA, FCM, IHC, WB |
| Target | CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Accession | P04626 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | A21 |
| Note | For research use only. |
Anti-Human ErbB2/Her2 Recombinant Antibody (A21)
Overview
Images
 Flow-cytometry |
Flow-cytometry using anti-human ERBB2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human ERBB2 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow-cytometry |
Flow-cytometry using anti-human ERBB2 antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ERBB2 antibody monoclonal antibody (Catalog # HY286307 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |