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Anti-Human MSLN/Mesothelin Recombinant Antibody (7D9)
Overview
Catalog No. HC496607
Brand abinScience
Host species Mouse
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications ELISA, FCM, WB
Target CAK1 antigen, MPF, MSLN, Megakaryocyte-potentiating factor, Mesothelin, Mesothelin, cleaved form, Pre-pro-megakaryocyte-potentiating factor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Accession Q13421
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID7D9
Note For research use only.
Images


SDS-PAGE
SDS-PAGE for Anti-Human MSLN/Mesothelin Antibody (7D9)

Bioactivity
Detects recombinant human MSLN (Catalog No: HC496011) in indirect ELISA

Flow cytometry
Flow-cytometry using anti-human MSLN antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MSLN monoclonal antibody (Catalog HC496607, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.