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Anti-Mouse CD25/IL2R alpha Recombinant Antibody (PC61/PC-61.5.3), FITC
Overview
Catalog No. MF996117
Brand abinScience
Host species Rat
Conjugation FITC
Tested applications FCM
Species reactivity Mouse
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.
Purity >90% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, lambda
Applications FCM
Target CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P01590
Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Clone IDPC61/PC-61.5.3
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images


Flow CytoMetry
Flow-cytometry using FITC anti-Mouse CD25 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD25 monoclonal antibody (Catalog: MF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.