Anti-Mouse CD20 Recombinant Antibody (MB20-11)
Overview
| Catalog No. |
MY257013 |
| Brand |
abinScience |
| Host species |
Mouse |
| Tested applications |
FCM |
| Species reactivity |
Mouse |
| Form |
Liquid |
| Storage buffer |
0.01M PBS pH 7.4. |
| Purity |
>95% as determined by SDS-PAGE. |
| Clonality |
Monoclonal |
| Isotype |
IgG2c, kappa |
| Applications |
ELISA, FCM, Functional assay, Research in vivo |
| Target |
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1 |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Endotoxin level |
< 10 EU/mg |
| Accession |
P19437 |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | MB20-11 |
| Product Usage Information |
| Application | Dilution |
|---|
| ELISA | 1:2000-1:20000 |
|
| Note |
For research use only |
Images
 Flow cytometry |
Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC conjugated anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience:MF690414) and cells analysed on a NovoCyte Flow Cytometer. |