| Catalog No. | HC273013 |
| Brand | abinScience |
| Host species | Rabbit |
| Tested applications | IF, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG |
| Applications | IF, FCM |
| Target | AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, CD69, CLEC2C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leukocyte surface antigen Leu-23, MLR-3 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | Q07108 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Clone ID | SRR0102 |
| Note | For research use only |
Anti-Human CD69 Recombinant Antibody (SRR0102)
Overview
Images
 Immunofluorescence |
Raji cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD69 (HC273013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD69 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |
 Flow cytometry |
Flow-cytometry using anti-Human CD69 antibody. Permeabilized Transfected cells and Control cells were stained with an anti-Human CD69 monoclonal antibody (Catalog HC273013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer. |