| Catalog No. | HB617043 |
| Brand | abinScience |
| Host species | Rabbit |
| Tested applications | IF, WB |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG |
| Applications | IF, WB |
| Target | BRAF, BRAF1, EC:2.7.11.1, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, p94, v-Raf murine sarcoma viral oncogene homolog B1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P15056 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Clone ID | SRR0002 |
| Note | For research use only |
Anti-Human B-Raf Recombinant Antibody (SRR0002)
Overview
Images
 Western blot |
Western blot analysis was performed using anti-B-Raf monoclonal antibody at 1 ug/mL on various samples. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK293T cell lysate |
 Immunofluorescence |
HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting B-Raf (HB617043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of B-Raf was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |