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Anti-Human PD-L1 Recombinant Antibody (SRR0417)
Overview
Catalog No. HV974133
Brand abinScience
Host species Rabbit
Tested applications IF, FCM, WB
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG
Applications IF, FCM, WB
Target B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, hPD-L1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession Q9NZQ7
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Clone IDSRR0417
Note For research use only
Images


Western blot		 Western blot analysis was performed using anti-PD-L1 monoclonal antibody at 1 ug/mL on various samples.
Lane 1: PD-L1-overexpressing HEK293T cell lysate

Immunofluorescence		 293T cells overexpressing PD-L1 were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting PD-L1 (HV974133) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of PD-L1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Flow cytometry		 Flow-cytometry using anti-Human PD-L1 antibody. Overexpression cells and Untransfected cells were stained with an anti-Human CD274 monoclonal antibody (Catalog HV974133, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.